LEMOLish is a motorized LEGO®-based light sheet microscope built to acquire scientific 3D images from centimeter-sized optically cleared biological samples. The system is open source, versatile, and costs about a hundred times less than existing custom/commercial solutions! This website summarizes the concepts of the project and provides access to the blueprint material (still under construction but fully available soon).
What's the catch?
LEMOLish relies on an innovative and compact optical design to create an array of thin light sheets without complex optical alignment. Combined with low noise affordable industrial CMOS cameras, the resulting image quality reaches scientific grade. No expensive medium-corrected objective lenses are required to acquire Z-stacks through the sample chamber thanks to the automated repositioning of the light sheet at the image plane. The images can be taken simultaneously from two detection sides (two cameras needed) and can be easily recombined so as to avoid typical deep-imaging image degradation. Altogether, the system achieves "stripe-free" 3D imaging at low cost and without tedious optical adjustments, expensive parts or complex image post-processing.
How does it work?
See all these functional parts working together
LEMOLish in action
Top view of LEMOLish during image acquisition: a 532nm laser lightsheet scanned through 5cm Glass chamber holding a large cleared specimen (>4cm chicken embryo)
Connect With Us (coming soon)
LEGO-based Motorized Lightsheet Microscope
LEMOLISH by Julien Colombelli, Sébastien Tosi is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at https://legolish.org/.
Permissions beyond the scope of this license may be available at https://github.com/adm-irb.